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Image Search Results
Journal: Food & Nutrition Research
Article Title: Urolithin A supplementation alleviates osteogenic disfunction and promotes bone fracture healing in inflammatory environments
doi: 10.29219/fnr.v70.13033
Figure Lengend Snippet: Sequences of primers for real-time quantitative PCR analysis
Article Snippet: The
Techniques: Real-time Polymerase Chain Reaction
Journal: Food & Nutrition Research
Article Title: Urolithin A supplementation alleviates osteogenic disfunction and promotes bone fracture healing in inflammatory environments
doi: 10.29219/fnr.v70.13033
Figure Lengend Snippet: UA alleviated pro-inflammation level and the M1 macrophage activation under TNF- α induced inflammation microenvironment. (a) CCK-8 assay was utilised to assess the cell viability of RAW264.7 of UA on 12, 24 and 48 h. (b, c) The concentration of IL-1 β and IL-6 in the medium of RAW264.7 after 12 h TNF- α stimulation with or without UA by ELISA. (d) The proteins of RAW264.7 were measured by western blot after 1 day and quantitative analysis. (e) The genes were measured by qRT-PCR after 1 day. (f) Immunofluorescence staining for iNOS after 1 day after TNF- α stimulation with or without UA. Scale bars, 100 μm. (g) The quantitative analysis of Immunofluorescence by image J. All of the experiments were independently accomplished by three times. * P < 0.05, ** P < 0.01 compared to the control group.
Article Snippet: The
Techniques: Activation Assay, CCK-8 Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Quantitative RT-PCR, Immunofluorescence, Staining, Control
Journal: Food & Nutrition Research
Article Title: Urolithin A supplementation alleviates osteogenic disfunction and promotes bone fracture healing in inflammatory environments
doi: 10.29219/fnr.v70.13033
Figure Lengend Snippet: UA accelerated TNF- α induced impaired bone fracture healing in a mice femur fracture model. (a) Experiment flow about animal study. (b) The representative μCT images of mouse femurs from the femur fracture + PBS, femur fracture + TNF- α and femur fracture + TNF- α + UA (50 mpk) group. (c) Graphic illustrations of BV/TV, Tb.N, Tb.Th and Tb.Sp in the indicated groups. (d) Histological section from femur fracture region stained with H&E and Masson staining. Scale bar, 500 μm. (e) Immunohistochemistry of COL1A1 and IL-1 β of femur fracture region. Scale bar, 500 μm. (f, g) The quantitative results of immunohistochemistry by image J. (h) Histological scores of three groups. All of the experiments were independently accomplished by five times. All error bars represent SDs. * P < 0.05, ** P < 0.01 compared to the control group.
Article Snippet: The
Techniques: Staining, Immunohistochemistry, Control